荷兰瓦赫宁根大学2023年招聘博士后（整合宿主-微生物组模型）

瓦格宁根大学，全称瓦格宁根大学与研究中心（英：Wageningen University & Research），位于荷兰瓦格宁根市，是荷兰的食品谷（Food Valley）的中心。瓦格宁根大学是一所的世界著名研究型大学，荷兰U类大学，其生态学、农业科学、生命科学、食品科学、环境科学等在全球享有极高的声誉，被公认为世界顶尖的农业和环境科学研究机构。

Postdoc integrated host-microbiome models

Wageningen University

Job description

Do you want to contribute to a better understanding of pig intestinal microbiome development during early life, and how this affects pig intestinal health and resilience to enteric infections and the need for antibiotics? Do you have experience with microbial ecology, host-microbiome interactions, organoids, immunology or systems biology? Then we are looking for you!

Wageningen University & Research is hiring a postdoctoral researcher (4 years, full-time employment) to become part of PIG-PARADIGM. In this project funded by the Novo Nordisk Foundation. researchers at the Laboratory of Microbiology, Host-Microbe Interactomics, Systems and Synthetic Biology and Wageningen Livestock Research join forces with teams at Aarhus University, the University of Copenhagen and Aalborg University in Denmark, and the University of California, Davis in the United States. We aim to decipher interactions of the developing pig, its diet and its intestinal microbiome, as well as the effect of post-weaning stress on intestinal mucosa functioning and susceptibility to infections. We want to turn that knowledge into new strategies for healthier pigs. We will study how members of the intestinal microbiome interact with each other and with the host intestine using advanced organoid-based models, and whether changes in the diet or the environment affect the intestinal microbiome so that less antibiotics are required. You will work in a team that is passionate about the role microbiomes play in the health of humans, animals and our environment.

Postdoc: Integrated host-microbiome models As postdoctoral researcher you will develop in vitro models of the intestinal microbe interaction in a hemi anaerobic culture system allowing oxygenation of intestinal organoid monolayers from the basal compartment and culture of selected bacteria and synthetic communities in a hypoxic apical chamber with a fluid control system. The model will be used to study the effects of the microbiota and specific metabolites on host innate defence and barrier function as well as epithelial permeability and infection with ETEC. You will also use organoid monolayers to model acute emotional stress at the time of weaning which activates the HPA axis, causing release of CRF in the lamina propria. The effects of this stress hormone on intestinal barrier, oxidative stress and infection with ETEC will be studied in organoid models combined with immune cells in the basal chamber. Moreover, the model will be adapted for use in the hemi-anaerobic culture system to identify microbes and metabolites which attenuate stress-induced barrier dysfunction and/or its inflammatory consequences.

Requirements

WHO ARE YOU? We are looking for a postdoctoral researcher that has:

a PhD in microbiology, molecular biology, cell biology, immunology or other relevant field;

knowledge of host-microbiome interactions;

experience with bioinformatics and biostatistics;

experience with molecular analyses of microbiomes and (anaerobic) microbial cultivation techniques;

experience with in vitro cell models and stem cell work;

a good track record or writing or contributing to scientific articles;

fluency in English (both spoken and written);

good communication and presentation skills;

the ability to work in international and multidisciplinary research teams.

, Conditions of employment

Wageningen University & Research offers excellent terms of employment. A few highlights from our Collective Labour Agreement include:

sabbatical leave, study leave, and partially paid parental leave;

working hours that can be discussed and arranged so that they allow for the best possible work-life balance;

the option to accrue additional compensation / holiday hours by working more, up to 40 hours per week;

there is a strong focus on vitality and you can make use of the sports facilities available on campus for a small fee;

a fixed December bonus of 8.3%;

excellent pension scheme.

A challenging position of 1.0 FTE for 24 months, starting Q1 2023, with the possibility to extend to 48 months. Depending on your experience, we offer a competitive gross salary of between