招聘简介：

英国埃克塞特

S66901博士后研究员/研究员

工程，数学和物理科学学院

生命系统研究所

这个全职岗位可以在固定期限内立即提供，最长可达35个月，以支持Frank Vollmer教授的工作。

您将结合Vollmer教授实验室开发的最先进的光学单分子技术应用您的专业知识来研究分子生物物理学。该技术将使您能够可视化酶等蛋白质的动态变化，并以前所未有的灵敏度和时间分辨率研究酶的反应机制和分子力学。这些基础和无标记的单分子研究将导致开发用于传感器芯片上的超敏感生物医学诊断的新型应用和分析工具。

这项工作将成为埃克塞特大学UKRI分子力学计划的重要组成部分。 UKRI资助的分子力学倡议是一项前沿研究计划，旨在实现我们如何在最终的单分子水平上检测，分析和操纵分子的突破。作为该计划的一部分，您将在一个更大的团队中工作，该团队开发新型激光传感，光学操作，分子力学分析和操作功能。这些功能将处于发展的最前沿，表明这些技术是传感器芯片上下一代支持分析和医疗保健技术的关键。我们的方法旨在揭示纳米机器的自然设计原则，并在更长的时间内实现合成生物分子机械和设计酶。

我们在寻找什么......

您是一名生物物理学家，在分子生物物理学和FRET，纳米等离子体/纳米孔等单分子技术的应用方面具有广泛的实验专业知识和良好的概念性理解。您曾与蛋白质和酶等生物分子及其功能化技术合作过。您可以定量分析和解释实验数据，并应用生物物理模型和理论来理解生物分子机制和构象动力学。

您很高兴能够成为由物理学家，生物学家和工程师组成的多学科团队的一员。您将在埃克塞特大学新成立的生命系统研究所工作，该研究所提供最先进的研究设施，以实现可视化和操纵生物分子机器动力学的首批光学方法之一。

参考

无标记光学检测单个酶 - 反应物反应和相关的构象变化

Science Advances 3（3），e1603044

Subramanian S，Wu H-Y，Constant T，Xavier J，Vollmer F.（2018）Label-Free Optical Single-Molecule Micro- and Nanosensors，Adv Mater，DOI：10.1002 / adma.201801246。

角色要求

丰富的分子生物物理实验和单分子技术，如FRET，纳米等离子体/纳米孔。熟悉构建单分子仪器或光学仪器是一个优点。

职责是进行研究并履行与该职位相关的其他主要职责和责任。您将能够提供有关研究进展和结果的信息，以口头，书面和电子方式传达复杂信息，并为外部机构准备提案和申请。

英文原文：

Exeter, United Kingdom

S66901 Postdoctoral Research Associate/Research Fellow

College of Engineering, Mathematics and Physical Sciences

Living Systems Institute

This full-time post is available immediately on a fixed term basis for up to 35 months to support the work of Professor Frank Vollmer.

You will apply your expertise in conjunction with the state-of-the-art optical single-molecule technique developed in Prof Vollmer’s Laboratory to study molecular biophysics. The technique will allow you to visualize the dynamics of proteins such as enzymes, and study reaction mechanisms and molecular mechanics of enzymes with an unprecedented sensitivity and time resolution. These fundamental and label-free single-molecule studies will lead into the development of novel applications and analytical tools for ultra-sensitive biomedical diagnostics on sensor chips.

The work will be the essential part of the UKRI Molecular Mechanics Initiative at the University of Exeter. The UKRI funded Molecular Mechanics Initiative is a cutting-edge research programme that aims to achieve breakthroughs in how we detect, analyse, and manipulate molecules at the ultimate single-molecule level. As part of the programme you will work in a larger team that develops novel laser sensing, optical manipulation, molecular mechanics analysis and manipulation capabilities. These capabilities will be at the forefront of developments showing that these techniques hold the key to the next generation of enabling analytical and healthcare technologies on sensor chips. Our approach aims to unravel nature’s design principles for nanomachines, and to realize synthetic biomolecular machinery and designer enzymes in the much longer term.

What we are looking for…

You are a biophysicist with extensive experimental expertise and sound conceptual understanding in molecular biophysics and the application of a single-molecule technique such as FRET, nanoplasmonics/ nanopores. You have worked with biomolecules such as proteins and enzymes and their functionalization techniques. You are able to analyse and interpret the experimental data quantitatively and apply biophysical models and theories to understand biomolecular mechanisms and conformational dynamics.

You are excited about working as part of a multi-disciplinary team composed of physicists, biologists and engineers. You will work at the newly established Living Systems Institute, University of Exeter which provides state-of-the-art research facilities for realizing one of the first optical approaches to visualize and manipulate the dynamics of biomolecular machines.

References

Label-free optical detection of single enzyme-reactant reactions and associated conformational changes

Science Advances 3 (3), e1603044

Subramanian S, Wu H-Y, Constant T, Xavier J, Vollmer F. (2018) Label-Free Optical Single-Molecule Micro- and Nanosensors, Adv Mater, DOI:10.1002/adma.201801246.

Requirements for the role

Extensive experimental expertise with molecular biophysics and a single molecule techniques such as FRET, nanoplasmonics/nanopores. Familiarity with building a single molecule instrumentation or optical instrumentation is a plus.

Duties are to undertake research and fulfil other main duties and accountability associated with the position. You will be able to present information on research progress and outcomes, communicate complex information, orally, in writing and electronically and prepare proposals and applications to external bodies.

Applicants will:

· Possess a PhD in Biophysics, Physics, Chemistry, Physical Chemistry, Biochemistry, Engineering, or a related discipline

· Be able to demonstrate sufficient knowledge in the discipline and of research methods and techniques to work within established research programmes

· Will be able to work collaboratively, supervise the work of others and act as team leader as required

· At PDRF level, will be a nationally recognized authority in molecular biophysics or single-molecule studies and will be able to develop research programmes and methodologies

You should demonstrate your suitability by submitting a CV and a personal statement addressing how you fulfil the requirements, which will be used to make an appointment at an appropriate level.

Please read our Job Description and Person Specification for full details of this role and candidate requirements at each level. Please indicate which level of appointment you are considering, within your application.

Appointments made at PDRA level, Grade E (£29,515 – £34,189 per annum) and at PDRF, Grade F (£35,211 – £39,609 per annum) based on qualifications and experience.

The University of Exeter

We are a member of the prestigious Russell Group of research-intensive universities and in the top 150 universities in the world (Times Higher Education World University Rankings 2019). We combine world-class teaching with world-class research, achieving a Gold rating in the Teaching Excellence Framework Award 2017.

With over 22,000 students and 5,500 staff from 180 different countries we offer a diverse and engaging environment in which to work. We are an equal opportunity employer, a Disability Confident employer and an Athena Swan accredited institution. Whilst all applicants will be judged on merit alone, we particularly welcome applications from groups currently underrepresented in the workforce.

Benefits

We offer some fantastic benefits including:

· 41 days leave

· options for flexible working

· sector leading policies around maternity, adoption and shared parental leave

· stunning campus environments in the beautiful South West of England

Further information

Please contact Professor Jennifer Littlechild (J.A.Littlechild@exeter.ac.uk; 01392 723468) or Professor Frank Vollmer (F.Vollmer@exeter.ac.uk; 01392 724771).

Closing date for applications is the 11 July.